English: (a) SCF contains three core subunits—the RING protein Rbx1, the cullin Cul1, and Skp1. Rbx1 binds the E2–ubiquitin conjugate. The target protein binds to an F-box protein that is bound to the enzyme core via interactions with the Skp1 subunit. After binding of a target protein to the F-box protein, the ubiquitin is transferred from E2 and attached via a peptide bond to a lysine side chain in the target protein. (b) A composite model structure for human SCF derived from X-ray structures of human Rbx1–Cul1–Skp1–Skp2 complex and the E2 enzyme Ubc7. The target protein (not shown here) interacts with the F-box protein Skp2, which thereby positions the substrate for ubiquitination by the E2 enzyme. Ubiquitin is not shown in this model but at the start of the reaction it would be bound to the E2 enzyme at the active-site cysteine shown in blue. (Adapted from Zheng, N. et al.: Nature 2002, 416:703–709.) (PDB 1fbv, 1ldk, 1fqr)^[1]