Summary
We show that a collection of 93 E. coli mutations which map between thr and leu and which block phage lambda DNA replication define two closely linked cistrons. Work published in the accompanying paper shows that these mutations also affect host DNA replication, so we designate them dnaJ and dnaK; the gene order is thr-dnaK-dnaJ-leu. Demonstration of two cistrons was possible with the isolation of lambda transducing phages carrying one or the other or both of the dna genes. These phages were employed in phage vs bacterial complementation studies which unambiguously show that dnaK and dnaJ are different cistrons.
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Bachmann, B.J.: Pedigrees of some mutant strains of Escherichia coli K-12. Bact. Rev. 36, 525–557 (1972)
Campbell, A.: Sensitive mutants of bacteriophage lambda. Virology 14, 22–32 (1961)
Davidson, N., Szybalski, W.: Physical and chemical characteristics of lambda DNA. The bacteriophage lambda (Hershey, A.D., ed.), pp. 45–82. New York: Cold Spring Harbor Laboratory 1971
Davis, B.D., Mingioli, E.S.: Mutants of Escherichia coli requiring methionine or vitamin B12. J. Bact. 60, 7–28 (1950)
Davis, R.W., Simon, M., Davidson, N.: Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids. Methods in enzymology (Grossman, L., ed.), 21, 413–428. New York and London: Academic Press 1968
Degnen, G.E., Cox, E.C.: Conditional mutator gene in Escherichia coli: isolation, mapping, and effector studies. J. Bact. 117, 477–487 (1974)
Feiss, M., Adhya, S., Court, D.L.: Isolation of plaque-forming, galactose-transducing strains of phage lambda. Genetics 71, 189–206 (1972)
Fowler, R.G., Degnen, G.E., Cox, E.C.: Mutational specificity of a conditional Escherichia coli mutator, mutD5. Molec. gen. Genet. 133, 179–191 (1974)
Franklin, N.: The N operon of lambda: extent and regulation as observed in fusions to the tryptophan operon of Escherichia coli. The bacteriophage lambda (A.D. Hershey, ed.), pp. 621–638. New York: Cold Spring Harbor Laboratory 1971
Georgopoulos, C.P.: A new bacterial gene (groPC) which affects lambda DNA replication. Molec. gen. Genet. 151, 35–39 (1977)
Georgopoulos, C.P., Herskowitz, I.: Escherichia coli mutants blocked in lambda DNA synthesis. The bacteriophage lambda (A.D. Hershey, ed.), pp. 553–564 New York: Cold Spring Harbor Laboratory 1971
Gottesman, M.E., Yarmolinsky, M.B.: Integration-negative mutants of bacteriophage lambda. J. molec. Biol. 31, 487–505 (1968)
Gottesman, S., Adhya, S.: Genetic, physical and restriction map of bacteriophage lambda. DNA insertion elements, plasmids and episomes (Bukari, A.I., Shapiro, J.A., Adhya, S.A., eds.), pp. 713–718. New York: Cold Spring Harbor Laboratory 1971
Low, B.: Formation of merodiploids in matings with a class of rec− recipient strains of Escherichia coli K12. Proc. nat. Acad. Sci. (Wash) 60, 160–167 (1968)
Murray, K., Murray, N.E.: Phage lambda receptor chromosomes for DNA fragments made with restriction endonuclease III of Haemophilus influenzae and restriction endonuclease I of Escherichia coli. J. molec. Biol. 98, 551–564 (1975)
Parkinson, J.S., Huskey, R.J.: Deletion mutants of bacteriophage lambda. I. Isolation and initial characterization. J. molec. Biol. 56, 369–384 (1971)
Saito, H., Uchida, H.: Initiation of the DNA replication of bacteriophage lambda in Escherichia coli K12. J. molec. Biol. 113, 1–25 (1977)
Saito, H., Uchida, H.: Organization and expression of dnaJ and dnaK genes of Escherichia coli K12. Molec. gen. Genet. 164, 1–8 (1978)
Sunshine, M., Feiss, M., Stuart, J., Yochem, J.: A new host gene (groPC) necessary for lambda DNA replication. Molec. gen. Genet. 151, 27–34 (1977)
Weisberg, R.A., Gallant, J.R.: Dual function of the lambda prophage repressor. J. molec. Biol. 25, 537–547 (1967)
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Yochem, J., Uchida, H., Sunshine, M. et al. Genetic analysis of two genes, dnaJ and dnaK, necessary for Escherichia coli and bacteriophage lambda DNA replication. Molec. Gen. Genet. 164, 9–14 (1978). https://doi.org/10.1007/BF00267593
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DOI: https://doi.org/10.1007/BF00267593
